Humane endpoints, body weight (g) at the beginning and at the end of the study, weight gain (%), average daily consumption of the food (g) and drink (mL) during the experimental trial (mean ± standard deviation)
Group | Humane endpoints | Body weight | Weight gain (%) | Average daily consumption | ||
---|---|---|---|---|---|---|
Initial (g) | Final (g) | Food (g) | Drink (mL) | |||
G1 WT water | 0.08 ± 0.18 | 29.65 ± 1.61 | 31.54 ± 3.29 | 6.24 ± 7.18 | 4.02 | 5.07 |
G2 HPV water | 0.36 ± 0.411 | 27.91 ± 3.19 | 28.69 ± 3.84 | 2.60 ± 3.65 | 4.24 | 6.46 |
G3 WT 0.013 g/mL | 0.24 ± 0.22 | 25.24 ± 1.27 | 26.72 ± 0.95 | 5.92 ± 2.19 | 4.26 | 5.18 |
G4 HPV 0.006 g/mL | 1.04 ± 0.092 | 23.83 ± 1.98 | 25.07 ± 2.00 | 3.12 ± 4.72 | 3.50 | 6.60 |
G5 HPV 0.008 g/mL | 0.36 ± 0.26 | 28.21 ± 4.73 | 28.99 ± 4.24 | 5.38 ± 6.74 | 4.29 | 8.01 |
G6 HPV 0.013 g/mL | 0.80 ± 0.40 | 24.44 ± 3.62 | 26.68 ± 3.89 | 9.24 ± 3.28 | 4.07 | 7.69 |
1 Statistically different from G4 (P < 0.05). 2 Statistically different from G5 (P < 0.05). G1 are wild-type (WT) mice that drink water; G2 are mice with human papillomavirus (HPV) that drank water; G3 are WT that drank daily an aqueous extract of Aloysia citrodora at a concentration of 0.013 g/mL; G4 are mice with HPV that drank daily an aqueous extract of A. citrodora at a concentration of 0.006 g/mL; G5 are mice with HPV that drank daily an aqueous extract of A. citrodora at a concentration of 0.008 g/mL; G6 are mice with HPV that drank daily an aqueous extract of A. citrodora at a concentration of 0.013 g/mL
BMF: Conceptualization, Investigation, Writing—original draft. AIFR, MJP, MJN, IG, MMSMB, and CV: Writing—review & editing. HV, CVN, JS, MGS, LG, MID, LB, and LF: Investigation, Writing—review & editing. RM and RMGDC: Writing—review & editing, Supervision. PAO: Conceptualization, Writing—review & editing, Supervision.
The authors declare that they have no conflicts of interest.
The experimental work was approved by the animal facility of the University of Trás-os-Montes e Alto Douro (UTAD) with the authorization of the Ethics Committee (approval no. 852-e-CITAB-2020_A_1-e- 122 CITAB-2021) and the Portuguese Veterinary Directorate (approval no. 014139).
Not applicable.
Not applicable.
The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.
Beatriz Medeiros-Fonseca has a Ph.D. grant with number [2020.07675.BD] (https://doi.org/10.54499/2020.07675.BD); Jéssica Silva has a Ph.D. grant with number [2020.07999.BD] (https://doi.org/10.54499/2020.07999.BD); Mónica G. Silva has a Ph.D. grant with number [UI/BD/151320/2021] (https://doi.org/10.54499/UI/BD/151320/2021) financed by FCT (Foundation for Science and Technology) through FSE (European Social Fund). This work is supported by National Funds by FCT-Portuguese under projects: CITAB [UID/04033/2020] (https://doi.org/10.54499/UIDB/04033/2020); CERNAS [UIDB/00681/2020] (https://doi.org/10.54499/UIDB/00681/2020); LEPABE [UIDB/00511/2020] (https://doi.org/10.54499/UIDB/00511/2020) and [UIDP/00511/2020] (https://doi.org/10.54499/UIDP/00511/2020); ALiCE [LA/P/0045/2020] (https://doi.org/10.54499/LA/P/0045/2020); CIMO [UIDB/00690/2020] (https://doi.org/10.54499/UIDB/00690/2020) and [UIDP/00690/2020] (https://doi.org/10.54499/UIDP/00690/2020); SusTEC, [LA/P/0007/2020] (https://doi.org/10.54499/LA/P/0007/2020); by national funds through the FCT/MCTES (PIDDAC). Furthermore, national funding by FCT/P.I., through the institutional scientific employment program contract for Lilian Barros, Maria I. Dias (https://doi.org/10.54499/CEECINST/00016/2018/CP1505/CT0004), Luís Félix (https://doi.org/10.54499/2021.00458.CEECIND/CP1690/CT0001) contract. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
© The Author(s) 2024.