Comparison of mean, SD, median, and quartiles of salivary hydroxyproline levels between two study groups
Salivary hydroxyproline levels (mg/L) group
Mean
SD
Median
1st quartile
3rd quartile
P-value
Healthy group
0.079
0.006
0.078
0.076
0.080
0.001
Unhealthy group
0.197
0.289
0.099
0.081
0.109
Declarations
Author contributions
MMM: Conceptualization, Investigation, Writing—review & editing, Supervision. HN: Investigation, Writing—original draft. FO: Conceptualization, Investigation, Writing—original draft. All authors read and approved the submitted version.
Conflicts of interest
All authors declare that they have no conflicts of interest.
Ethical approval
This study was approved by the ethics committee of Birjand University of Medical Sciences with code IR.BUMS.REC.1402.071.
Consent to participate
Parents filled out the informed consent before sampling and clinical exams of their children. In addition, the child’s parents were assured of the confidentiality of their information and the non-disclosure of information individually.
Consent to publication
Not applicable.
Availability of data and materials
The data are available based on the eligible request to the corresponding author.
Al-Ghutaimel H, Riba H, Al-Kahtani S, Al-Duhaimi S. Common periodontal diseases of children and adolescents.Int J Dent. 2014;2014:850674. [DOI] [PubMed] [PMC]
Singh O, Reddy VK, Sharma L, Pradhan D, Srivastava R. Association of gingivitis with children oral health-related quality of life in Lucknow: A cross-sectional study.J Family Med Prim Care. 2020;9:1177–81. [DOI] [PubMed] [PMC]
Trombelli L, Farina R, Silva CO, Tatakis DN. Plaque-induced gingivitis: Case definition and diagnostic considerations.J Periodontol. 2018;89:S46–73. [DOI] [PubMed]
Ji S, Choi YS, Choi Y. Bacterial invasion and persistence: critical events in the pathogenesis of periodontitis?J Periodontal Res. 2015;50:570–85. [DOI] [PubMed]
Chandran A, Bhandary R, Shenoy N, Shetty UA. Analysis of collagen fibers in human gingival tissues using picrosirius red stain under polarized microscope.J Indian Soc Periodontol. 2021;25:106–11. [DOI] [PubMed] [PMC]
Bartold PM, Walsh LJ, Narayanan AS. Molecular and cell biology of the gingiva.Periodontol 2000. 2000;24:28–55. [DOI] [PubMed]
Preethanath RS, Ibraheem WI, Anil A. Pathogenesis of gingivitis. In: Sridharan G, Sukumaran A, Al Ostwani AEO, editors. Oral Diseases. Rijeka: IntechOpen; 2020. [DOI]
Kinane DF. Causation and pathogenesis of periodontal disease.Periodontol 2000. 2001;25:8–20. [DOI] [PubMed]
Jenkins CL, Bretscher LE, Guzei IA, Raines RT. Effect of 3-hydroxyproline residues on collagen stability.J Am Chem Soc. 2003;125:6422–7. [DOI] [PubMed]
Greabu M, Battino M, Mohora M, Totan A, Didilescu A, Spinu T, et al. Saliva—a diagnostic window to the body, both in health and in disease.J Med Life. 2009;2:124–32. [PubMed] [PMC]
Dawes C. Physiological factors affecting salivary flow rate, oral sugar clearance, and the sensation of dry mouth in man.J Dent Res. 1987;66:648–53. [DOI] [PubMed]
Fey JMH, Bikker FJ, Hesse D. Saliva Collection Methods Among Children and Adolescents: A Scoping Review.Mol Diagn Ther. 2024;28:15–26. [DOI] [PubMed] [PMC]
Ravi V, Ponnaiyan D, Prakash P. Hydroxyproline Levels in Gingiva and GCF in Patients with and without Chronic Periodontitis.Int J Dentistry Oral Sci. 2021;8:4908–11. [DOI]
Angelova S, Salim A, Kiselova-Kaneva Y, Ivanova D, Andreeva-Borisova R, Peev S. The role and significance of some salivary amino acids in periodontal diseases, including plaque-induced gingivitis—a literature review.Scr Sci Med Dent. 2021;7:7–17. [DOI]
Mahfouz M, Masri I, Mahfouz H, Mahfouz Y. Correlation between Vitamin C Deficiency and Hydroxyproline Amino Acid in Young Children of Northern Part in Palestine.Open J Pediatr. 2015;5:151–5. [DOI]
Rath S, Das D, Sahoo SK, Raj A, Guddala NR, Rathee G. Childhood dental fear in children aged 7-11 years old by using the Children’s Fear Survey Schedule-Dental Subscale.J Med Life. 2021;14:45–9. [DOI] [PubMed] [PMC]
Gonçalves Lda R, Soares MR, Nogueira FCS, Garcia C, Camisasca DR, Domont G, et al. Comparative proteomic analysis of whole saliva from chronic periodontitis patients.J Proteomics. 2010;73:1334–41. [DOI] [PubMed]
Kejriwal S, Bhandary R, Thomas B, Kumari S. Estimation of levels of salivary mucin, amylase and total protein in gingivitis and chronic periodontitis patients.J Clin Diagn Res. 2014;8:ZC56–60. [DOI] [PubMed] [PMC]
Löe H, Silness J. Periodontal Disease in Pregnancy I. Prevalence and Severity.Acta Odontol Scand. 1963;21:533–51. [DOI] [PubMed]
Akalin FA, Sengün D, Eratalay K, Renda N, Cağlayan G. Hydroxyproline and total protein levels in gingiva and gingival crevicular fluid in patients with juvenile, rapidly progressive, and adult periodontitis.J Periodontol. 1993;64:323–9. [DOI] [PubMed]
Koss MA, Castro CE, Salúm KM, Kishimoto E, Takagi S, López ME. Gingival crevicular fluid biomarkers in patients with gingivitis and chronic periodontitis.J Hard Tissue Biol. 2010;19:111–6. [DOI]
Koss MA, Castro CE, Salúm KM, López ME. Changes in saliva protein composition in patients with periodontal disease.Acta Odontol Latinoam. 2009;22:105–12. [PubMed]
Meyer MS, Joshipura K, Giovannucci E, Michaud DS. A review of the relationship between tooth loss, periodontal disease, and cancer.Cancer Causes Control. 2008;19:895–907. [DOI] [PubMed] [PMC]
Shi F, Liu W, Yao Y, Zhang Q, Chen Z, Xian Y, et al. Predictive salivary biomarkers for early diagnosis of periodontal diseases—current and future developments.TJB. 2023;48:335–44. [DOI]
Chauhan VS, Chauhan RS, Devkar N, Vibhute A, More S. Gingival and Periodontal Diseases in Children and Adolescents.J Dent Allied Sci. 2012;1:26–9. [DOI]
Pari A, Ilango P, Subbareddy V, Katamreddy V, Parthasarthy H. Gingival diseases in childhood—a review.J Clin Diagn Res. 2014;8:ZE01–4. [DOI] [PubMed] [PMC]
Ketabi M, Tazhibi M, Mohebrasool S. The Prevalance and Risk Factors of Gingivitis Among the Children Referred to Isfahan Islamic Azad University (Khorasgan Branch) Dental School, In Iran.J Dent Res. 2008;3.
Jahanimoghadam F, Shamsaddin H. The prevalence and risk factors of gingivitis in a population of 6-year-old children in Iran.J Oral Health Oral Epidemiol. 2016;5:129–33.
Funna MA, Zakaria R, Maidar M, Abdullah A, Hermansyah H. Determinant Causes of Periodontal Diseases on Students of Faculty of Public Health in Banda Aceh and Aceh Besar.IJMMU. 2023;10:49. [DOI]
Sharva V, Reddy V, Bhambal A, Agrawal R. Prevalence of Gingivitis among Children of Urban and Rural Areas of Bhopal District, India.J Clin Diagn Res. 2014;8:ZC52–4. [DOI] [PubMed] [PMC]
Pourhashemi SJ, Motlagh MG, Jahed Khaniki GR. Prevalence and Intensity of Gingivitis among 6–10 Years Old Elementary School Children in Tehran, Iran.J Med Sci. 2007;7:830–4. [DOI]
