The authors would like to thank Dr. Noha Azab lecturer of the Department of Oral Medicine and Periodontology, at the Faculty of Dentistry, Cairo University for her help in the clinical part and follow-up with the patients.
Author contributions
GN: Investigation, Data curation, Writing—original draft, Resources. FMZ: Conceptualization, Investigation, Data curation, Writing—review & editing, Supervision. AE and AF: Methodology, Writing—original draft. DG: Validation, Writing—original draft, Writing—review & editing. BE: Conceptualization, Investigation, Writing—original draft, Writing—review & editing, Methodology. All authors reviewed the results and approved the final version of the manuscript.
Conflicts of interest
The authors declare no conflicts of interest to report regarding the present study.
Ethical approval
The study was performed in accordance with the Declaration of Helsinki, and the protocol was approved by the Ethics Committee of Scientific Research, Faculty of Dentistry, Cairo University (approval No. 17 8 3). The aim of the study was explained to all subjects participating in this study and written informed consents were obtained before enrolment.
Consent to participate
The informed consent to participate in the study was obtained from all participants.
Consent to publication
Not applicable.
Availability of data and materials
The datasets used and/or analyses used during the current study are available from the corresponding author on reasonable request.
Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.
References
Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012.Int J Cancer. 2015;136:E359–86. [DOI] [PubMed]
Speight PM, Khurram SA, Kujan O. Oral potentially malignant disorders: risk of progression to malignancy.Oral Surg Oral Med Oral Pathol Oral Radiol. 2018;125:612–27. [DOI] [PubMed]
Zahran FM, Elsaadany B, Azab NA, El-Gawish A, Ghalwash D. Dysplasia in Oral Lichen Planus in a Sample of Egyptians Attending 2 Tertiary Care Centers in Cairo.Oral Dis. 2024. [DOI] [PubMed]
Mello FW, Miguel AFP, Dutra KL, Porporatti AL, Warnakulasuriya S, Guerra ENS, et al. Prevalence of oral potentially malignant disorders: A systematic review and meta-analysis.J Oral Pathol Med. 2018;47:633–40. [DOI] [PubMed]
Warnakulasuriya S, Ariyawardana A. Malignant transformation of oral leukoplakia: a systematic review of observational studies.J Oral Pathol Med. 2016;45:155–66. [DOI] [PubMed]
Lodi G, Porter S. Management of potentially malignant disorders: evidence and critique.J Oral Pathol Med. 2008;37:63–9. [DOI] [PubMed]
Lodi G, Franchini R, Warnakulasuriya S, Varoni EM, Sardella A, Kerr AR, et al. Interventions for treating oral leukoplakia to prevent oral cancer.Cochrane Database Syst Rev. 2016;7:CD001829. [DOI] [PubMed] [PMC]
Chau L, Jabara JT, Lai W, Svider PF, Warner BM, Lin HS, et al. Topical agents for oral cancer chemoprevention: A systematic review of the literature.Oral Oncol. 2017;67:153–9. [DOI] [PubMed]
Wang J, Xie T, Wang B, William WN Jr, Heymach JV, El-Naggar AK, et al. PD-1 Blockade Prevents the Development and Progression of Carcinogen-Induced Oral Premalignant Lesions.Cancer Prev Res (Phila). 2017;10:684–93. [DOI] [PubMed] [PMC]
Al-Attass SA, Zahran FM, Turkistany SA. Nigella sativa and its active constituent thymoquinone in oral health.Saudi Med J. 2016;37:235–44. [DOI] [PubMed] [PMC]
Yaghutian Nezhad L, Mohseni Kouchesfahani H, Alaee S, Bakhtari A. Thymoquinone ameliorates bleomycin-induced reproductive toxicity in male Balb/c mice.Hum Exp Toxicol. 2021;40:S611–21. [DOI] [PubMed]
Alaee S, Mirani M, Derakhshan Z, Koohpeyma F, Bakhtari A. Thymoquinone improves folliculogenesis, sexual hormones, gene expression of apoptotic markers and antioxidant enzymes in polycystic ovary syndrome rat model.Vet Med Sci. 2023;9:290–300. [DOI] [PubMed] [PMC]
Padhye S, Banerjee S, Ahmad A, Mohammad R, Sarkar FH. From here to eternity—the secret of Pharaohs: Therapeutic potential of black cumin seeds and beyond.Cancer Ther. 2008;6:495–510. [PubMed] [PMC]
Banerjee S, Padhye S, Azmi A, Wang Z, Philip PA, Kucuk O, et al. Review on Molecular and Therapeutic Potential of Thymoquinone in Cancer.Nutr Cancer. 2010;62:938–46. [DOI] [PubMed] [PMC]
Younus H. Molecular and Therapeutic actions of Thymoquinone: Actions of Thymoquinone. Springer Nature; 2018. [DOI]
Gali-Muhtasib H, Ocker M, Kuester D, Krueger S, El-Hajj Z, Diestel A, et al. Thymoquinone reduces mouse colon tumor cell invasion and inhibits tumor growth in murine colon cancer models.J Cell Mol Med. 2008;12:330–42. [DOI] [PubMed] [PMC]
Fatfat Z, Fatfat M, Gali-Muhtasib H. Therapeutic potential of thymoquinone in combination therapy against cancer and cancer stem cells.World J Clin Oncol. 2021;12:522–43. [DOI] [PubMed] [PMC]
Al-Attas SA, Munshi A, Noorwali AW, Algrigri MA, Abohager EA, Zahran FM. Nigella Sativa Extract Chemoprevention in Oral Cancer: in vivo study.Adv In Environmental Biol. 2015;9:75–90.
Nagao T, Warnakulasuriya S, Nakamura T, Kato S, Yamamoto K, Fukano H, et al. Treatment of oral leukoplakia with a low-dose of beta-carotene and vitamin C supplements: A randomized controlled trial.Int J Cancer. 2015;136:1708–17. [DOI] [PubMed]
Ma C, Liu C, Ahmed AF, Niu Y, Kang W. Optimum Extraction Technology for the Seed Oil of Nigella sativa L.J Food Qual. 2019. [DOI]
Aragón-Sánchez J, Quintana-Marrero Y, Aragón-Hernández C, Hernández-Herero MJ. ImageJ: A Free, Easy, and Reliable Method to Measure Leg Ulcers Using Digital Pictures.Int J Low Extrem Wounds. 2017;16:269–73. [DOI] [PubMed]
Ghalwash DM, El-Gaaly K, Zahran FM, Shaker O, El-Fol HA. The diagnostic and prognostic value of salivary sCD44 level determination in oral malignant and potentially premalignant lesions.Adv Environ Biol. 2012;6:302–10.
Ghalwash DM. Diagnostic and prognostic value of salivary biomarkers in oral cancer and precancer: Review article.J Oral Maxillofac Surgery Med Pathol. 2020;32:538–43. [DOI]
Warnakulasuriya S. Oral potentially malignant disorders: A comprehensive review on clinical aspects and management.Oral Oncol. 2020;102:104550. [DOI] [PubMed]
Khader M, Bresgen N, Eckl PM. Antimutagenic effects of ethanolic extracts from selected Palestinian medicinal plants.J Ethnopharmacol. 2010;127:319–24. [DOI] [PubMed]
Arafa MG, Ghalwash D, El-Kersh DM, Elmazar MM. Propolis-based niosomes as oromuco-adhesive films: A randomized clinical trial of a therapeutic drug delivery platform for the treatment of oral recurrent aphthous ulcers.Sci Rep. 2018;8:18056. [DOI] [PubMed] [PMC]
Svider PF, Warner BM, Schwartz SJ, Garshott DM, Weinstein JD, Fribley AM. Mucosal Therapy for Potentially Malignant Diseases and OSCC. In: Fribley AM, editor. Targeting Oral Cancer. Cham: Springer; 2016. pp. 283–99. [DOI]
Abdel-Aziz AM, AbouShousha A, Ali S, Zahran F. Prevalence of Potentially Malignant Lesions and Oral Cancer Among Smokers in an Egyptian cohort: A Hospital-based Cross-Sectional Study.Adv Dent J. 2020;2:93–100. [DOI]
Rahman M, Beg S, Kumar V, Ahmad FJ. Nanomedicine for Bioactives: Healthcare applications. Springer Singapore; 2020. [DOI]
Mu HQ, Yang S, Wang YJ, Chen YH. [Role of NF-κB in the anti-tumor effect of thymoquinone on bladder cancer].Zhonghua Yi Xue Za Zhi. 2012;92:392–6. Chinese. [DOI] [PubMed]
Paramasivam A, Sambantham S, Shabnam J, Raghunandhakumar S, Anandan B, Rajiv R, et al. Anti-cancer effects of thymoquinone in mouse neuroblastoma (Neuro-2a) cells through caspase-3 activation with down-regulation of XIAP.Toxicol Lett. 2012;213:151–9. [DOI] [PubMed]
Peng L, Liu A, Shen Y, Xu HZ, Yang SZ, Ying XZ, et al. Antitumor and anti-angiogenesis effects of thymoquinone on osteosarcoma through the NF-κB pathway.Oncol Rep. 2013;29:571–8. [DOI] [PubMed]
Chu SC, Hsieh YS, Yu CC, Lai YY, Chen PN. Thymoquinone Induces Cell Death in Human Squamous Carcinoma Cells via Caspase Activation-Dependent Apoptosis and LC3-II Activation-Dependent Autophagy.PLoS One. 2014;9:e101579. [DOI] [PubMed] [PMC]
Shahraki S, Khajavirad A, Shafei MN, Mahmoudi M, Tabasi NS. Effect of total hydroalcholic extract of Nigella sativa and its n-hexane and ethyl acetate fractions on ACHN and GP-293 cell lines.J Tradit Complement Med. 2015;6:89–96. [DOI] [PubMed] [PMC]
Alaufi OM, Noorwali A, Zahran F, Al-Abd AM, Al-Attas S. Cytotoxicity of thymoquinone alone or in combination with cisplatin (CDDP) against oral squamous cell carcinoma in vitro.Sci Rep. 2017;7:13131. [DOI] [PubMed] [PMC]
Singla AK, Chawla M, Singh A. Potential Applications of Carbomer in Oral Mucoadhesive Controlled Drug Delivery System: A Review.Drug Dev Ind Pharm. 2000;26:913–24. [DOI] [PubMed]
Neetha MC, Panchaksharappa MG, Pattabhiramasastry S, Shivaprasad NV, Venkatesh UG. Chemopreventive Synergism between Green Tea Extract and Curcumin in Patients with Potentially Malignant Oral Disorders: A Double-blind, Randomized Preliminary Study.J Contemp Dent Pract. 2020;21:521–31. [DOI] [PubMed]
Singh M, Krishanappa R, Bagewadi A, Keluskar V. Efficacy of oral lycopene in the treatment of oral leukoplakia.Oral Oncol. 2004;40:591–6. [DOI] [PubMed]
Benner SE, Winn RJ, Lippman SM, Poland J, Hansen KS, Luna MA, et al. Regression of Oral Leukoplakia With α-Tocopherol: A Community Clinical Oncology Program Chemoprevention Study.J Natl Cancer Inst. 1993;85:44–7. [DOI] [PubMed]
Silverman S Jr, Gorsky M, Lozada F. Oral leukoplakia and malignant transformation. A follow-up study of 257 patients.Cancer. 1984;53:563–8. [DOI] [PubMed]