Table Info

Table 2.

Primers sequences used for PCR amplification

Gene	Primers sequences (5–3)	Tm (°C)	PCR product size (bp)
β-globin	GH20-F 5'-GAAGAGCCAAGGACAGGTAC-3'	54	268
β-globin	PC04-R 5'-CAACTTCATCCACGTTCACC-3'	54	268
HPV	MY09-F 5'-CGTCCMARRGGAWACTGATC-3'	55	450
	MY11-R 5'-GCMCAGGGWCATAAYAATGG-3'	55	450
	GP5⁺-F 5'-TTTGTTACTGTGGTAGATACTAC-3'	42	150
	GP6⁺-R 5'-GAAAAATAAACTGTAAATCATATTC-3'	42	150
EBV	EBNA-2 OP-F 5'-GCGGGTGGAGGGAAAGG-3'	58	168
	EBNA-2 OP-R 5'-GTCAGCCAAGGGACGCG-3'	58	168
	EBNA-2 IP-F 5'-AGGCTGCCCACCCTGAGGAT-3'	66	168
	EBNA-2 IP-R 5'-GCCACCTGGCAGCCCTAAAG-3'	66	168
SV40	SVTAGP1-F 5'-TTAGCAATTCTGAAGGAAAGTCCTTG-3'	52	126
SV40	SVTAGP3-F 5'-ACCTGTTTTGCTCAGAAG-3'	52	126
MMTV	MMTV1-F 5'-TGCGCCTTCCCTGACCAAGGG-3'	54	356
MMTV	MMTV2-R 5'-GTAACACAGGCAGATGTAGG-3'	54	356

F: forward primer; R: reverse primer; PCR: polymerase chain reaction; Tm: melting temperature; HPV: human papillomavirus; EBV: Epstein-Barr virus; MMTV: mouse mammary tumor virus; SV40: Simian virus 40. M = A + C, R = A + G, W= A + T, Y = C + T

Declarations

Acknowledgments

Special acknowledgment for support of Faculty of Sciences and Techniques, Mohammedia, University Hassan II of Casablanca and Ministry of National Education, Vocational Training, Higher Education and Scientific Research in Morocco.

Author contributions

KAT: Conceptualization, Data curation, Methodology, Formal analysis, Software, Investigation, Project administration, Writing—original draft. AS: Writing—review & editing, Project administration. SAS: Visualization, Validation. KN: Investigation. MB: Resources. MME: Project administration, Supervision. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The study protocol was approved by the Ethics Committee for Biomedical Research of the Faculty of Medicine and Pharmacy of Casablanca, Morocco (Reference 3/2018 on 30.04.2018).

Consent to participate

Free and informed consent was obtained from all patients involved in this research and the confidentiality of their personal information was well-respected according to ethical rules.

Consent to publication

Not applicable.

Availability of data and materials

The datasets generated and/or analyzed in the course of the present study are available from the corresponding author upon reasonable request.

Funding

Not applicable.

Copyright

Publisher’s note

Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.

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