Table Info

Table 1.

Primers used for detection of β-globin and VDR gene polymorphisms

Genes	Primers	Sequences	Annealing temperature (°C)	Amplified fragment size (bp)	Reference
β-globin gene	PC04	5'-CAACTTCATCCACGTTCACC-3'	54	268	[34]
β-globin gene	GH20	5'-GAAGAGCCAAGGACAGGTAC-3'	54	268	[34]
VDR gene SNP (rs1544410) restricted by BsmI	Forward	5'-CAACCAAGACTACAAGTACCGCGTCAGTGA-3'	56	825	[35]
VDR gene SNP (rs1544410) restricted by BsmI	Reverse	5'-AACCAGCGGGAAGAGGTCAAGGG-3'	56	825	[35]
VDR gene SNP (rs7975232)/(rs731236) restricted by ApaI/TaqI	Forward	5'-CAGAGCATGGACAGGGAGCAA-3'	66	740	[36]
	Reverse	5'-GCAACTCCTCATGGCTGAGGTCTC-3'	66	740	[36]

VDR: vitamin D receptor; SNP: single nucleotide polymorphism

Declarations

Acknowledgments

The authors would like to thank Hassan II University of Casablanca, Faculty of Sciences and Techniques—Mohammedia, the members of the team of Virology, Oncology and Biotechnologies, Laboratory of Virology, Oncology, Biosciences, Environment and New Energies. As well as the Laboratory of Research and Biosafety of Mohammed V Construction Teaching Hospital in Rabat, for their help during sample collection.

Author contributions

KN: Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Writing—original draft. AL: Resources, Writing—review & editing. IT: Data curation, Investigation. KAT: Investigation, Methodology. MM and AA: Resources. KE: Data curation, Resources. MB: Supervision, Writing—review & editing. MME: Conceptualization, Data curation, Formal analysis, Investigation, Supervision, Validation, Writing—review & editing. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The study protocol was approved by the Ethics Committee for Biomedical Research of the Faculty of Medicine and Pharmacy of Casablanca, Morocco (No. 3/2018/April 30, 2018).

Consent to participate

The informed consent to participate in the study was obtained from all participants.

Consent to publication

Not applicable.

Availability of data and materials

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

Funding

Not applicable.

Copyright

Publisher’s note

Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.

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