QTOF–MS retention time, mass, and empirical formula of the flavonoids in the new compound
Name
Retention time (RT)
Mass
Empirical molecular formula
Quercetin 3-sambubioside-3’-glucoside
0.943
757.1827
C32H38O21
Apigenin-7-4’alloside
1.555
636.1671
C29H32O16
Epicatechin-epigallocatechin-3-O-gallate
1.882
746.1441
C37H30O17
Kaempferol 7-O-glucoside
4.574
448.1008
C21H20O11
Hesperetin
12.212
302.0799
C16H14O6
Luteolin
14.375
286.0476
C15H10O6
Quercetin
15.264
302.0426
C15H10O7
Declarations
Acknowledgments
The authors thank Mrs. Belgin Berker Budak for her support for this research. We also thank Mrs. Begüm Buğdaycı Açıkkol, Director of Kobay Exper. Animals Inc., Turkey.
Author contributions
GGB conducted this study and wrote this paper with SÖ. MB edited this manuscript. GGB did the compound formulation and HPLC analysis. TŞ performed LC–MS–MS analysis. BM applied QTOF analysis. ŞSM did total phenolic and flavonoid content determination. CÇÇ proceeded In vitro cytotoxicity and genotoxicity tests: In vivo acute/sub-acute oral toxicity and LD50 studies. OT performed In vivo acute/sub-acute oral toxicity and LD50 studies. MB assessed the tissue samples microscopically. SÖ made Statistical analysis. All authors have read and agreed to the published version of the manuscript.
Conflicts of interest
The authors declare no conflicts of interest.
Ethical approval
Institutional Review Board statement: the animal experiments were approved by the regional Ethical Committee for animal research. The licensing ethical committee approved the experiments, including any relevant details before the animal experiments (27.11.2020/514). The authors confirm that all experiments were performed in accordance with relevant international guidelines and regulations. The authors also complied with the ARRIVE guidelines. All manipulations were made to minimize animal suffering and reduce the number of animals used.
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