Table Info

Table 2.

Efficiencies, amplicon length and sequences of qPCR primers used for quantification of murine transcripts

Gene name	Efficiency (%)	Amplicon length	Forward sequence (5’–3’)	Reverse sequence (5’–3’)	NCBI accession
HSPA9	92	112 bp	GGGCAAACAAGCAAAGGTCC	TTGCCGTTTTGCTGGCATAC	NM_010481
GNPDA1	142	170 bp	GGACGCCACCTTGGAACTAA	GAATACCACGTCCTGGCACA	NM_011937
ATP6V1E1	102	87 bp	ATAGCCCAGCAGATGATGCC	TCCTCCTGAAGCTCAGTCCA	NM_007510
LOX	103	165 bp	CAGGAACCGACCTGGATACG	GCCCTATATGCTGAACTGGC	NM_010728
GUSB	91	108 bp	CAGCGGCTGGGCTTTTTA	CGCTTGCCCTCAACCAAGTT	NM_010368

NCBI: National Center for Biotechnology Information

Supplementary materials

The supplementary material for this article is available at: https://www.explorationpub.com/uploads/Article/file/1002201_sup_1.pdf.

Declarations

Acknowledgments

We would like to thank Andrada Tarta (Department of Biomedical Sciences, University of Guelph), for her valuable contribution to the optimization of the adipocyte differentiation protocol at the A.V.P lab.

Author contributions

CMVS: Conceptualization, Investigation, Formal analysis, Validation, Visualization, Writing—original draft, Writing—review & editing. GK: Conceptualization, Investigation, Formal analysis, Writing—review & editing. NKP, EE and AVVC: Investigation, Formal analysis. SLC and AMVP: Conceptualization, Methodology, Writing—review & editing, Supervision, Project administration, Funding acquisition. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

The data are available from the authors upon request (sherri.christian@mun.ca; aviloria@uoguelph.ca).

Funding

This work was funded by a Cancer Research Society operating grant to SC and AVP [22130]; an Internal Faculty of Science, Memorial University of Newfoundland award to SC; a Natural Sciences and Engineering Research Council (NSERC) of Canada grant to AVP [#401248]; CMVS was supported by an Art Rouse Memorial Master of Science scholarship from the Ontario Veterinary College (OVC); GK was supported by a Carol Ann Cole Comfort Heart Summer Studentship via the Beatrice Hunter Cancer Research Institute and is supported by a CGS-M award from the Canadian Institutes of Health Research (CIHR). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

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