Advantages and disadvantages of different tissue dissociation methods
| Tissue dissociation methods | Advantages | Disadvantages |
|---|---|---|
| Enzymatic [8–10] | -Extraction of a sufficient quantity of required cells.-Accessibility and widespread application.-Relatively easy removal of stromal cells and fibroblasts. | Requires the selection of specific enzymes for each type of tumor tissue. |
| Chemical [11, 12] | -Allows better preservation of phenotypic and genetic characteristics.-Cells divide rapidly when cultivated. | The complexity of the procedure, with the efficiency not fully established. |
| Mechanic [8–13] | Simplicity and accessibility, with no chemical impact on cells. | -Formation of a large number of non-viable cells.-Release of cell contents with damaged membranes into the external environment, and sample contamination. |
VVP: Writing—original draft, Writing—review & editing. ZEG, SSI, AAR, and VVS: Writing—review & editing.
The authors declare that they have no conflicts of interest.
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This paper has been supported by the Russian Science Foundation grant [21-74-10021]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
© The Author(s) 2024.