Pros and cons of analysis methods used in liquid biopsies
Analysis method
Pros
Cons
qPCR
Good sensitivity and specificityWidely available
Limited to HTS and discovery studies
ddPCR
High sensitivity
Expensive
BEAming
Detection of ctDNA with very low mutant allele frequency
Expensive
NGS
High sensitivity
Bioinformatics support required
Identification of several miRNA variants
No automation
Whole-genome sequencing
Wide application
Expensive
Time-consuming
Bioinformatics support required
CellSearch
FDA approved
Expensive
Good sensitivity and reproducibility
Limited to CTCs with high EpCAM levels
False positives due to inflammation
Declarations
Author contributions
AP and IRS contributed conception and design of the manuscript. AP, IRS, MVAO, JR, RMM, AR and MT wrote sections of the manuscript. All authors contributed to manuscript revision, read and approved the submitted version.
Conflict of interest
The authors declare that they have no conflicts of interest.
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