Concordance of BRAF mutational status established by RT-PCR and NGS methods
RT-PCR
Total
BRAF mutated
BRAF wild-type
NGS
BRAF mutated
12
2
14
BRAF wild-type
0
15
15
Total
12
17
29
Kappa = 0.89, 95% confidence interval (CI) 0.68 ± 1.05. Concordance samples are in bold.
Declarations
Author contributions
PC, LA and CT designed the study; MM, AG, MG, EG, AA collected data and organized the database; MM, PJ, MG, EG performed the experiments; PC, PJ, AA, CT analyzed and interpreted data; PC performed the statistical analysis and wrote the first draft of the manuscript; All authors contributed to manuscript revision, read and approved the submitted version.
Conflicts of interest
The authors declare that they have no conflicts of interest.
Ethical approval
The study was approved by Internal Review Board of the Hospital Clinic of Barcelona (Barcelona, Spain; HCB/2017/0097).
Consent to participate
The informed consent to participate in the study was obtained from all participants.
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