Summary of the comparison between ISTH and CLSI guidelines for LA detection
Category
ISTH 2020
CLSI 2014
Sample preparation
Double centrifugation
Double centrifugation
Assay to use
dRVVT and APTT
dRVVT and APTT and/or others
Testing order
Screening => mixing & confirmatory
Screening => confirmatory => mixing
Calculations for PL-dependence
Normalized ratio or percentage correction
Normalized ratio or percentage correction
Mixing test
Perform on: mixture with NPP
Interpret with MTC
Perform on: mixture with NPP
Interpret with MTC or ICA
MTC: mixing test-specific cut-off; ICA: index of circulating anticoagulant
Declarations
Author contributions
OK: Conceptualization, Investigation, Writing—original draft, Writing—review & editing. MP: Investigation, Writing—review & editing. JA: Supervision, Validation, Writing—review & editing. All authors read and approved the submitted version.
Conflicts of interest
OK was an employee of HYPHEN BioMed at the time the work carried out. MP is an employee of HYPHEN BioMed. JA is a consultant for HYPHEN BioMed and Sysmex Corporation.
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