Table Info

Table 1.

RNA quantification of samples subjected to different protocols and resuspended in TRI-reagent or RLT buffer extracted by RNeasy Mini kit (Qiagen, Hilden, Germany) or TRI-reagent (Sigma-Aldrich)

Samples	Concentration (ng/μL)	Absorbance (260/280 nm)
3-step UC
PBS + RLT (AllPrep kit)	4.1	2.19
	2.4	3.05
	1.1	3.08
TRI-reagent + PBS	782.4	1.78
	1,045.1	1.82
	1,185.0	1.58
RLT (AllPrep kit)	7.7	3.05
TRI-reagent	1,281.9	1.71
WW
TRI-reagent + PBS	1,087.6	1.71
	1,321.9	1.71
	1,281.9	1.64
PBS + RLT (AllPrep kit)	1.0	1.62
RLT (AllPrep kit)	0.8	0.12
TRI-reagent	486.1	1.43
CDP
RLT (3-step, AllPrep kit)	13.5	1.45
RLT (WW, AllPrep kit)	0.6	3.05

CDP: contaminant-depleted plasma; PBS: phosphate buffered saline; UC: ultracentrifugation; WW: samples without washing

Declarations

Acknowledgments

We kindly thank the participants and the staff of TransplantLines for providing plasma material of pre transplanted patients.

TransplantLines Investigators: Coby Annema, Stefan P. Berger, Hans Blokzijl, Frank A.J.A. Bodewes, Marieke T. de Boer, Kevin Damman, Martin H. de Borst, Arjan Diepstra, Gerard Dijkstra, Rianne M. Douwes, Caecilia S.E. Doorenbos, Michele F. Eisenga, Michiel E. Erasmus, C. Tji Gan, Antonio W. Gomes Neto, Eelko Hak, Bouke G. Hepkema, Marius C. van den Heuvel, Frank Klont, Tim J. Knobbe, Daan Kremer, Coretta van Leer-Buter, Henri G.D. Leuvenink, Marco van Londen, Willem S. Lexmond, Vincent E. de Meijer, Hubert G.M. Niesters, Gertrude J. Nieuwenhuis-Moeke, L. Joost van Pelt, Robert A. Pol, Robert J. Porte, Adelta V. Ranchor, Jan Stephan F. Sanders, Marion J. Siebelink, Riemer J.H.J.A. Slart, J. Cas Swarte, Daan J. Touw, Charlotte A. te Velde-Keyzer, Erik A.M. Verschuuren, Michel J. Vos, Rinse K. Weersma, Stephan J.L. Bakker

Author contributions

MMA: Formal analysis, Investigation, Writing—original draft, Writing—review & editing. MCTA: Formal analysis, Investigation, Writing—review & editing. JCW: Formal analysis, Writing—review & editing. MBH and FVV: Writing—review & editing. All members of TransplantLines: Resources. HB and HM: Conceptualization, Supervision, Writing—review & editing. All authors have read and agreed to the published version of the manuscript.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

TransplantLines Cohort and Biobank Study (ClinicalTrials.gov identifier: NCT02811835) was approved by the Medical Ethics Committee (METc 2014/077).

Consent to participate

Informed consent to participate in the study was obtained from all participants.

Consent to publication

Not applicable.

Availability of data and materials

The raw data supporting the conclusions of this manuscript, including western blot analyses, will be made available by the authors, without undue reservation, to any qualified researcher.

Funding

The TransplantLines Biobank and Cohort study was supported by grants from Astellas BV (project code: [TransplantLines Biobank and Cohort study]), Chiesi Pharmaceuticals BV (project code: [PA-SP/PRJ-2020-9136]), and NWO/TTW via a partnership program with DSM, Animal Nutrition and Health, The Netherlands (project code: [14939]). The project was co-financed by the Dutch Ministry of Economic Affairs and Climate Policy by means of so-called PPP-allowances, made available by the Top Sector Life Sciences & Health to stimulate public-private partnerships (project code: [PPP-2019-032] and [PPP-2022-015]). The funders had no role in the study design, data collection, analysis, reporting, or the decision to submit for publication.

Copyright

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