Table Info

Table 1.

Location of splice sites and sequences responsible for generating various spliced HBV pgRNA

5’ donor splice site nucleotide position^*	Splice site sequence (based on HBV genotype D)	Splice variants produced by splicing at this splice site
2067/8	CAG/GCAAGC	Sp2, Sp3, Sp8, Sp10, Sp12, Sp15, Sp16, Sp17
2087/8	GGG/GTGAGT (genotype B and C only)	Sp4, Sp5
2447/8	AAT/GTTAGT	Sp1, Sp2, Sp4, Sp7, Sp8, Sp9, Sp10, Sp12, Sp13, Sp15
2471/2	AAG/GTGGGR	Sp6, Sp11, Sp16, Sp18
2985/6	AAG/GTAGGA	Sp7, Sp8, Sp14, Sp18, Sp19, Sp20
3’ acceptor splice site nucleotide position^*	Splice site sequence (based on HBV genotype D)	Splice variants produced by splicing at this splice site
2234/5	GAA/G	Sp12, Sp15
2349/50	TAG/A	Sp2, Sp4, Sp8, Sp10, Sp16
2901/2	CAG/T	Sp7, Sp8, Sp13, Sp18
281/2	TAG/G	Sp9, Sp10, Sp11, Sp12, Sp17, Sp20
488/9	CAG/G	Sp1, Sp2, Sp3, Sp4, Sp5, Sp6, Sp7, Sp8, Sp14, Sp15, Sp16, Sp18

^* Numbering is based on EcoR1 start site. HBV: hepatitis B virus; C: cytosine; A: adenine; G: guanine; T: thymine; R: adenine or guanine

Declarations

Author contributions

LCM: Investigation, Writing—original draft, Writing—review & editing. ML: Writing—review & editing, Supervision. PAR: Conceptualization, Writing—review & editing, Supervision.

Conflicts of interest

PAR has received investigator-initiated industry-funded grants from Gilead. None of this support is relevant to the work in this manuscript. The remaining authors declare no conflicts of interest.

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

Not applicable.

Funding

LCM, ML and PAR were funded by National Health and Medical Research Council (NHMRC) grant [1145977]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

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