Description of dyes for MitoTracker Green FM and MitoTracker Deep Red FM

DyeManufacturerTarget organelleExcitation wavelengthEmission wavelengthStockDilution used
MitoTracker Green FMThermo Fischer Scientific (catalog No. M7514)Mitochondria490 nm516 nm50 μg in 1 mL DMSO300 nM (diluted in pre-warmed DMEM)
MitoTracker Deep Red FMThermo Fischer Scientific (catalog No. M22426)Mitochondria644 nm665 nm50 μg in 1 mL DMSO500 nM (diluted in pre-warmed DMEM)

DMEM: Dulbecco’s modified eagle medium; DMSO: dimethylsulfoxide