Table Info

Table 3.

Body weight (g) changes in SD rats during subacute toxicity studies for 90 days

Rats	Treatment (days)	Control	Free ISL	Excipient	ISL@ZLH NPs
Rats	Treatment (days)	Control	Free ISL	Excipient	27.5 mg/kg	55.0 mg/kg	110.0 mg/kg
Male	1	127.92 ± 2.95	127.14 ± 3.53	127.16 ± 3.35	126.60 ± 5.27	127.98 ± 5.56	131.40 ± 4.49
	10	182.10 ± 2.47	181.22 ± 3.38	180.88 ± 2.68	181.00 ± 5.27	182.38 ± 5.95	185.52 ± 4.74
	20	244.66 ± 1.59	243.24 ± 2.00	241.54 ± 5.19	237.28 ± 7.07	248.66 ± 14.30	249.48 ± 6.82
	30	294.76 ± 2.98	295.54 ± 5.20	292.16 ± 6.67	284.34 ± 9.65	300.64 ± 17.47	301.30 ± 6.36
	40	339.54 ± 2.22	341.56 ± 2.30	339.32 ± 5.39	336.54 ± 8.17	350.80 ± 13.68	347.24 ± 6.57
	50	370.60 ± 0.82	372.06 ± 3.48	369.32 ± 5.37	372.14 ± 5.96	381.90 ± 11.25	377.92 ± 7.43
	60	408.94 ± 2.05	410.00 ± 4.79	406.86 ± 4.70	411.66 ± 5.20	418.40 ± 9.28	414.32 ± 7.14
	70	459.06 ± 3.42	459.54 ± 5.72	457.66 ± 3.47	459.58 ± 4.01	467.04 ± 7.17	460.68 ± 6.43
	80	476.32 ± 3.22	476.98 ± 5.41	475.80 ± 2.66	477.92 ± 4.83	485.10 ± 6.03	479.76 ± 5.57
	90	499.08 ± 2.51	499.36 ± 6.13	497.78 ± 3.42	500.48 ± 5.31	506.58 ± 5.33	503.92 ± 5.03
Female	1	117.26 ± 1.80	112.62 ± 7.08	113.90 ± 2.49	111.14 ± 2.54	116.56 ± 6.49	118.86 ± 5.67
	10	144.16 ± 2.15	139.40 ± 6.64	139.34 ± 2.38	136.08 ± 2.24	141.66 ± 7.17	145.20 ± 6.41
	20	168.58 ± 4.87	161.16 ± 6.83	167.26 ± 4.37	161.90 ± 2.62	168.38 ± 10.08	167.44 ± 7.97
	30	203.18 ± 7.61	193.42 ± 6.73	203.62 ± 3.68	192.86 ± 3.24	201.62 ± 10.61	198.64 ± 9.06
	40	250.20 ± 8.02	240.74 ± 5.49	251.40 ± 7.72	240.04 ± 5.13	250.06 ± 10.16	248.84 ± 9.36
	50	268.64 ± 7.67	260.54 ± 5.27	269.48 ± 8.03	261.62 ± 5.04	268.96 ± 8.48	269.74 ± 8.40
	60	283.16 ± 6.86	276.06 ± 5.30	283.94 ± 6.31	277.70 ± 5.02	282.68 ± 7.08	284.78 ± 7.52
	70	300.38 ± 7.03	294.16 ± 5.63	302.30 ± 6.12	295.38 ± 4.92	299.52 ± 6.64	302.32 ± 8.11
	80	307.62 ± 6.09	303.02 ± 5.47	309.16 ± 5.34	303.30 ± 4.72	307.34 ± 6.43	309.88 ± 6.46
	90	320.30 ± 4.19	317.14 ± 4.91	321.78 ± 4.11	315.08 ± 4.63	320.20 ± 4.26	322.80 ± 6.39

Values are expressed as mean ± standard deviation, n = 5. The Dunnett t-test was used to determine the significance of data variability between the treated and control groups. Statistical significance was considered at ^*P < 0.05

Declarations

Author contributions

KY and KG equally contributed to: Conceptualization, Methodology, Validation, Investigation, Writing—original draft, Writing—review & editing. FG and CX: Methodology, Software, Validation, Formal analysis, Data curation. JC: Supervision, Project administration, Funding acquisition. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

The research protocol received approval from the Ethics Committee of the Affiliated Hospital of Chengdu University of Traditional Chinese Medicine (20220421).

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

The data that support the findings of this study are available from the corresponding author upon reasonable request.

Funding

This work was supported by the Midstream Research Programme for Universities from the Innovation and Technology Commission of Hong Kong [MRP/027/18X]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

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