Variation of the experimental hydrophobicity index (HI) using as separation mode acetonitrile (% ACN) in RP HPLC 0.1 % of formic acid for modified (acetylated) and non-modified peptide pairs

Acetylated lysine residues in peptide (number of peptide pairs)Experimental hydrophobicity index (ΔHI) in RP HPLC 0.1% formic acid (% ACN)Δ(Ac)LysΔΔHI/Δ(Ac)Lys
                    0 (10,632)                                  5.02      -       -
                    1 (13,791)                                  9.14      1     4.12
                    2 (2,390)                                  11.20      2     6.18
                    3 (316)                                  12.54      3     7.52