Table Info

Table 2.

Characterization and cytotoxic effect of hybrid peptides in HeLa, Ca Ski, and L929 cells after 2 h treatment at 37°C

Code	Sequence	RP-HPLC		ESI-MS (M)		IC₅₀ (μM)
Code	Sequence	t_R (min)	Purity (%)	Theorical	Experimental	HeLa	Ca Ski	L929
1	RWQWRWQWR-Ahx-RRWQWR	7.0	96.1	2,567.37	2,567.40	21.6	28.6	40.2
2	RRWQWR-Ahx-RWQWRWQWR	6.9	96.3	2,567.37	2,567.37	26.7	48.9	55.5
3	RWQWRWQWR-Ahx-RLLRRLLR	7.8	97.8	2,675.59	2,675.61	13.3	16.8	30.7
4	RLLRRLLR-Ahx-RWQWRWQWR	7.0	97.4	2,675.59	2,675.60	16.0	22.4	42.4
5	RWQWRWQWR-Ahx-QQLPSSSTSTYP	7.0	97.7	2,875.44	2,875.46	> 69.5	ND	ND
6	RWQWRWQWR-Ahx-GDALFSVPLEVY	8.3	99.3	2,889.50	2,889.55	> 69.2	ND	ND
7	RWQWRWQWR-Ahx-QVNGLGERSQQM	7.0	98.2	2,926.48	2,926.52	> 68.3	ND	ND
8	RWQWRWQWR-Ahx-KQNLAEG	6.8	99.2	2,339.23	2,339.25	> 85.4	ND	ND
9	RWQWRWQWR-Ahx-YGRKKRPQRRR	6.5	99.1	3,080.74	3,080.76	19.4	34.3	23.6
10	RWQWRWQWR-Ahx-RRRRRRRR	6.4	98.5	2,847.66	2,847.70	12.8	55.5	22.3
11	RWQWRWQWR-Ahx-RKKRRQRRR	6.3	98.3	2,919.70	2,919.72	17.2	67.7	25.0
12	RKKRRQRRR-Ahx-RWQWRWQWR	6.4	97.3	2,919.70	2,919.68	17.1	16.4	20.2
13	RWQWRWQWR-Ahx-KLALKLALK	7.5	98.5	2,577.54	2,577.60	12.0	71.7	> 77.5
14	KLALKLALK-Ahx-RWQWRWQWR	7.2	99.2	2,577.54	2,577.54	15.1	34.6	30.9
15	KWQWKWQWK-Ahx-RRWQWR	6.8	91.8	2,483.35	2,483.38	14.6	19.6	6.7
16	RWQWRWQWR-Ahx-KKWQWK	6.9	98.4	2,483.35	2,483.38	15.7	74.6	> 80.5
17	KWQWKWQWK-Ahx-KKWQWK	6.7	98.2	2,399.33	2,399.36	23.9	35.1	16.5
18	(RWQWRWQWR)₂K-Ahx-RRWQWR	7.3	93.1	4,164.2	4,164.24	9.6	36.0	35.6

ND: not determined; Ahx: 6-aminohexanoic acid (magenta); RP-HPLC: reverse phase-high performance liquid chromatography; ESI-MS: electrospray ionization-mass spectrometry

Supplementary materials

The supplementary materials for this article are available at: https://www.explorationpub.com/uploads/Article/file/100864_sup_1.pdf.

Declarations

Author contributions

NAC: Conceptualization, Methodology, Investigation, Formal analysis, Writing—original draft, Visualization, Writing—review & editing. JEGC: Conceptualization, Methodology, Formal analysis, Resources, Writing—original draft, Visualization. CMPG: Formal analysis, Resources, Writing—original draft. YVC: Formal analysis, Resources, Writing—original draft. ACBC: Formal analysis, Resources, Writing—original draft. RFM: Conceptualization, Supervision. ZJRM: Conceptualization, Methodology, Resources, Writing—review & editing. JERM: Conceptualization, Methodology, Writing—review & editing. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

Not applicable.

Consent to participate

Not applicable.

Consent to publication

Not applicable.

Availability of data and materials

The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.

Funding

This research was conducted with the financial support of MinCiencias with the project: “Obtención de un prototipo peptídico promisorio para el desarrollo de un medicamento de amplio espectro para el tratamiento del cáncer de colon, cuello uterino y próstata”. Contract RC No. 845-2019. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

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