The primary amino acid sequences of Ala scan peptides were listed with the inhibition rate at a fixed concentration of 1 μM. Those with > 90% inhibition were further characterized for IC50
Name
Amino acid sequence
Inhibition (%)
IC50 ± SD (nM)
BCP-8B
Ala-Cys-Ala-Gly-Arg-Cys-Pro-Ser-Ala-Cys-Leu
93.8%
153.63 ± 17.87 nM
BCP-9B
Ala-Cys-Ala-Ala-Arg-Cys-Pro-Ser-Ala-Cys-Leu
66.2%
-
BCP-10B
Ala-Cys-Ala-Gly-Ala- Cys-Pro-Ser-Ala-Cys-Leu
46.6%
-
BCP-11B
Ala-Cys-Ala-Gly-Arg-Cys-Ala-Ser-Ala-Cys-Leu
81.8%
-
BCP-12B
Ala-Cys-Ala-Gly-Arg-Cys-Pro-Ala-Ala-Cys-Leu
69.7%
-
BCP-13B
Ala-Cys-Ala-Gly-Arg-Cys-Pro-Ser-Ala-Cys-Ala
3.4%
-
BCP-14B
Ala-Cys-Gly-Gly -Arg-Cys-Pro-Ser-Ala-Cys-Leu
10.4%
-
BCP-15B
Ala-Cys-Ala-Pro-Arg-Cys-Pro-Ser-Ala-Cys-Leu
99.5%
62.00 ± 7.31 nM
BCP-16B
Ala-Cys-Ala-Gly-Arg-Cys-Gly-Ser-Ala-Cys-Leu
95.8%
116.67 ± 7.79 nM
-: no data. SD: standard deviation; IC50: half-maximal inhibitory concentration
We acknowledge the instrument facility of the Biotech Drug Research Center for providing support with mass spectrometry, protein expression, and plate reader. We also acknowledge the institutional center for Shared Technologies and Facilities of SIMM for providing services including NMR and MS data recording.
Author contributions
QW: Conceptualization, Validation, Formal analysis, Investigation, Data curation, Writing—original draft. YW and JL: Conceptualization, Data curation, Supervision. HL and SC: Conceptualization, Resources, Supervision, Writing—original draft, Writing—review & editing, Project administration, Funding acquisition, Resources. All authors read and approved the submitted version.
Conflicts of interest
The authors declare that they have no conflicts of interest.
Ethical approval
Not applicable.
Consent to participate
Not applicable.
Consent to publication
Not applicable.
Availability of data and materials
The raw data supporting the conclusions of this manuscript will be made available by the corresponding author, without undue restriction, to any qualified researcher upon request.
Funding
This study was supported by the Distinguished Young Scholars Program and the General Program of the National Natural Science Foundation of China (#22477128) awarded to SC. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
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