Table Info

Table 1.

Dimeric peptides characterization by RP-HPLC and LC-MS

Code	Sequence	RP-HPLC		Monoisotopic mass
Code	Sequence	t_R (min)	Purity (%)	Theoretical	Experimental
²⁶[F]	(RRWQWRFKKLG)₂-K-Ahx	5.9	99	3,341.964	3,341.9736
1	(^dRRWQWRFKKLG)₂-K-Ahx	6.0	88	3,341.964	3,342.2998
2	(Orn-RWQWRFKKLG)₂-K-Ahx	5.9	84	3,257.995	3,258.7513
3	(R-Orn-WQWRFKKLG)₂-K-Ahx	5.8	90	3,257.995	3,257.9099
4	(RRWQW-Orn-FKKLG)₂-K-Ahx	5.8	99	3,257.995	3,257.7685
5	(^dR-Orn-WQWRFKKLG)₂-K-Ahx	5.9	84	3,257.995	3,256.8235
6	(^dRRWQW-Orn-FKKLG)₂-K-Ahx	5.9	86	3,257.995	3,257.8924
7	(Ahx-RRWQWRFKKLG)₂-K-Ahx	5.9	83	3,568.132	3,568.1213
8	(Biotin-Ahx-RRWQWRFKKLG)₂-K-Ahx	6.1	90	4,021.452	4,020.2752
9	(CH₃CO-Ahx-RRWQWRFKKLG)₂-K-Ahx	6.3	86	3,652.142	3,652.1590
10	(RWQWRWQWR)₂-K-Ahx	7.2	99	3,195.670	3,196.6716
11	(Ahx-RWQWRWQWR)₂-K-Ahx	7.1	99	3,422.838	3,422.8376
12	(RWQWRWQW-Orn)₂-K-Ahx	7.2	97	3,111.642	3,111.6287
13	(CH₃CO-RWQWRWQWR)₂-K-Ahx	7.4	91	3,280.713	3,280.6926

LC-MS: liquid chromatography-mass spectrometry; RP-HPLC: reverse phase-high performance liquid chromatography; t_R: retention time

Supplementary materials

The supplementary figures and table for this article are available at: https://www.explorationpub.com/uploads/Article/file/100891_sup_1.pdf.

Declarations

Author contributions

KJCM: Conceptualization, Methodology, Investigation, Formal analysis, Writing—original draft, Visualization, Writing—review & editing. JERC, YVC, and LFOG: Formal analysis, Resources, Writing—original draft. CMPG: Formal analysis, Resources, Writing—original draft. ACBC: Formal analysis, Resources. RFM: Conceptualization, Supervision, Funding acquisition. JELM: Conceptualization, Methodology, Writing—review & editing. ZJRM: Conceptualization, Methodology, Resources, Writing—review & editing, Funding acquisition. JEGC: Conceptualization, Methodology, Formal analysis, Resources, Writing—original draft, Visualization, Funding acquisition. All authors read and approved the submitted version.

Conflicts of interest

The authors declare that they have no conflicts of interest.

Ethical approval

For hemolytic activity (code 05-2019) and Irwin test (code 03-2018) were approved by the institutional ethics committee of the National University of Colombia. Zebrafish experiments were approved by the Institutional Committee for Animal Care and Use of the University of the Andes, code 24-01-2020.

Consent to participate

Informed consent to participate in the study was obtained from all participants.

Consent to publication

Not applicable.

Availability of data and materials

The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.

Funding

This research was conducted with the financial support of MinCiencias with the projects: “Obtención de un prototipo peptídico promisorio para el desarrollo de un medicamento de amplio espectro para el tratamiento del cáncer de colon, cuello uterino y próstata”. Contract RC No. [845-2019]. “Desarrollo de un medicamento contra el cáncer de mama basado en un péptido polivalente derivado de la LfcinB: Estudio de la fase preclínica (fase cero), caracterización fisicoquímica de un lote del fármaco para estudios preclínicos”. RC No. [706-2018]. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Copyright

Publisher’s note

Open Exploration maintains a neutral stance on jurisdictional claims in published institutional affiliations and maps. All opinions expressed in this article are the personal views of the author(s) and do not represent the stance of the editorial team or the publisher.

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