PL composition of DL and HL sunflower lecithins by 31P NMR
PL type
Percentage of total PL
PL/L (g/100 g)
DL
HL
DL
HL
PC
36
17.98
22.98
12.5
1-LPC
nd
1.58
nd
0.74
2-LPC
1.2
17.52
0.53
8.15
PI
31.6
27.36
21.89
20.63
LPI
nd
nd
nd
nd
PS-Na
0.5
nd
0.33
nd
PE
15.4
10.56
9.27
6.91
LPE
1.2
8.3
0.48
3.52
N-aPE
1.5
nd
1.22
nd
PG
1.8
nd
1.14
nd
DPG
0.7
nd
0.41
nd
PA
9.1
3.8
5.15
2.35
LPA
0.4
5.68
0.13
2.2
Other PLs
0.5
7.23
0.32
5.03
Total
100
100
63.85
62.03
Coefficient of variation < 4%. L: lecithin average values of two replicates; nd: no signal assignment; LPI: lysophosphatidylinositol; PS-Na: phosphatidylserine-Na; N-aPE: n-acyl-PE; PG: phosphatidylglycerol; DPG: diphosphatidylglycerol
Declarations
Acknowledgments
The authors wish to thank the donation of chia seed oil sunflower lecithins (Lasenor Emul S.L., Spain). The authors are also grateful to Flavio Accinelli (Bio Esanco S.A., Argentina), Mariela Fernández, and her working group (CETMIC, Argentina) for their technical assistance, particularly in measuring emulsion stability and zeta potential, respectively.
The authors declare that they have no conflicts of interest.
Ethical approval
Not applicable.
Consent to participate
Not applicable.
Consent to publication
Not applicable.
Availability of data and materials
Data from the present manuscript will be made available upon request.
Funding
This work was supported by Universidad Nacional de La Plata (UNLP) [11/X907]; Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) [PIP 2007]; Agencia Nacional de Promoción Científica y Tecnológica [PICT 2020-1274, PICT 2019-01775]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Ixtaina VY, Martínez ML, Spotorno V, Mateo CM, Maestri DM, Diehl BWK, et al. Characterization of chia seed oils obtained by pressing and solvent extraction.J Food Compos Anal. 2011;24:166–74. [DOI]
Derbyshire E. Micronutrient intakes of British adults across mid-life: a secondary analysis of the UK National Diet and Nutrition Survey.Front Nutr. 2018;5:55. [DOI] [PubMed] [PMC]
Elagizi A, Lavie CJ, Marshall K, DiNicolantonio JJ, O’Keefe JH, Milani RV. Omega-3 polyunsaturated fatty acids and cardiovascular health: a comprehensive review.Progress Cardio Diseas. 2018;61:76–85. [DOI] [PubMed]
Layé S, Nadjar A, Joffre C, Bazinet RP. Anti-inflammatory effects of omega-3 fatty acids in the brain: physiological mechanisms and relevance to pharmacology.Pharmacol Rev. 2018;70:12–38. [DOI] [PubMed]
Manson JE, Cook NR, Lee IM, Christen W, Bassuk SS, Mora S, et al. Marine n−3 fatty acids and prevention of cardiovascular disease and cancer.New Eng J Med. 2019;380:23–32. [DOI] [PubMed] [PMC]
Simopoulos AP. An increase in the omega-6/omega-3 fatty acid ratio increases the risk for obesity.Nutr. 2016;8:128. [DOI] [PubMed] [PMC]
Simopoulos AP. The importance of the ratio of omega-6/omega-3 essential fatty acids.Biomed Pharmac. 2002;56:365–79. [DOI] [PubMed]
Julio LM, Copado CN, Crespo R, Diehl BWK, Ixtaina VY, Tomás MC. Design of microparticles of chia seed oil by using the electrostatic layer-by-layer deposition technique.Powder Technol. 2019;345:750–57. [DOI]
da Silva Marineli R, Lenquiste SA, Moraes ÉA, Maróstica MR Jr. Antioxidant potential of dietary chia seed and oil (Salvia hispanica L.) in diet-induced obese rats.Food Res Int. 2015;76:666–74. [DOI] [PubMed]
de Souza T, Vargas da Silva S, Fonte-Faria T, Nascimento-Silva V, Barja-Fidalgo C, Citelli M. Chia oil induces browning of white adipose tissue in high-fat diet-induced obese mice.Mol Cell Endocr. 2020;507:110772. [DOI] [PubMed]
Gazem RAA, Chandrashekariah SA. Pharmacological properties of Salvia hispanica (chia) seeds: a review.J Crit Rev. 2016;3:63–7.
Tan C, McClements DJ. Application of advanced emulsion technology in the food industry: a review and critical evaluation.Foods. 2021;10:812. [DOI] [PubMed] [PMC]
McClements DJ, Jafari SM. General aspects of nanoemulsions and their formulation. In: Jafari SM, McClements DJ, editors. Nanoemulsions. Massachusetts: Academic Press; 2018. pp. 3–20.
Pathak M. Nanoemulsions and their stability for enhancing functional properties of food ingredients. In: Oprea AE, Grumezescu AM, editors. Nanotechnology applications in food. Massachusetts: Academic Press; 2017. pp. 87–106.
Lee L, Norton IT. Comparing droplet breakup for a high-pressure valve homogeniser and a Microfluidizer for the potential production of food-grade nanoemulsions.J Food Eng. 2013;114:158–63. [DOI]
Kavinila S, Nimbkar S, Moses JA, Anandharamakrishnan C. Emerging applications of microfluidization in the food industry.J Agric Food Res. 2023;12:100537. [DOI]
Julio LM, Copado CN, Diehl BWK, Ixtaina VY, Tomás MC. Chia bilayer emulsions with modified sunflower lecithins and chitosan as delivery systems of omega-3 fatty acids.LWT. 2018;89:581–90. [DOI]
McClements DJ, Bai L, Chung C. Recent advances in the utilization of natural emulsifiers to form and stabilize emulsions.Annu Rev Food Sci Technol. 2017;8:205–36. [DOI] [PubMed]
Gutiérrez-Méndez N, Chavez-Garay DR, Leal-Ramos MY. Lecithins: a comprehensive review of their properties and their use in formulating microemulsions.J Food Biochem. 2022;46:e14157. [DOI] [PubMed]
Acosta E. Bioavailability of nanoparticles in nutrient and nutraceutical delivery.Curr Opin Colloid In. 2009;14:3–15. [DOI]
Limam Z, Selmi S, Sadok S, El Abed A. Extraction and characterization of chitin and chitosan from crustacean by-products: biological and physicochemical properties.Afr J Biotechnol. 2011;10:640–47.
Wani TA, Masoodi FA, Jafari SM, McClements DJ. Safety of nanoemulsions and their regulatory status. In: Jafari SM, McClements DJ, editors. Nanoemulsions. Massachusetts: Academic Press; 2018. pp. 613–28.
Müller RH, Gohla S, Keck CM. State of the art of nanocrystals – special features, production, nanotoxicology aspects and intracellular delivery.Eur J Pharmac Biopharmac. 2011;78:1–9. [DOI] [PubMed]
Capitani MI, Sandoval‐Peraza M, Chel‐Guerrero LA, Betancur‐Ancona DA, Nolasco SM, Tomás MC. Functional chia oil-in-water emulsions stabilized with chia mucilage and sodium caseinate.J Am Oil Chem Soc. 2018;95:1213–21. [DOI]
Julio LM, Ixtaina VY, Fernández MA, Sánchez RMT, Wagner JR, Nolasco SM, et al. Chia seed oil-in-water emulsions as potential delivery systems of ω-3 fatty acids.J Food Eng. 2015;162:48–55. [DOI]
Julio LM, Ixtaina VY, Fernández M, Torres Sánchez RM, Nolasco SM, Tomás MC. Development and characterization of functional O/W emulsions with chia seed (Salvia hispanica L.) by-products.J Food Sci Technol. 2016;53:3206–14. [DOI] [PubMed] [PMC]
Pereyra-Castro SC, Pérez-Pérez V, Hernández-Sánchez H, Jiménez-Aparicio A, Gutiérrez-López GF, Alamilla-Beltrán L. Effect of composition and homogenization pressure of chia oil emulsions elaborated by microfluidization.Rev Mex Ing Quim. 2019;18:69–81. [DOI]
Teng J, Hu X, Wang M, Tao N. Fabrication of chia (Salvia hispanica L.) seed oil nanoemulsions using different emulsifiers.J Food Process Preserv. 2018;42:e13416. [DOI]
Maldonado A, Riquelme N, Muñoz-Fariña O, García O, Arancibia C. Stability and bioaccessibility of α-tocopherol-enriched nanoemulsions containing different edible oils as carriers.LWT. 2023;174:114419. [DOI]
Fernandes SS, Bernardino JCC, Owen PQ, Prentice C, Salas-Mellado MDLM, Segura-Campos MR. Effect of the use of ethanol and chia mucilage on the obtainment and techno-functional properties of chia oil nanoemulsions.J Food Process Preserv. 2021;45:e15181. [DOI]
Paquot C, Hautffenne A. Standard methods for the analysis of oils, fats and derivates. 7th ed. Oxford: Blackwell Scientific Publications; 1992.
Diehl B. NMR spectroscopy of natural substances. In: Holzgrabe U, Wawer I, Diehl B, editors. NMR spectroscopy in pharmaceutical analysis. Elsevier; 2008. pp. 181–200.
Miadonye A, Amadu M. Theoretical interpretation of pH and salinity effect on oil-in-water emulsion stability based on interfacial chemistry and implications for produced water demulsification.Processes. 2023;11:2470. [DOI]
Ciron CIE, Gee VL, Kelly AL, Auty MAE. Comparison of the effects of high-pressure microfluidization and conventional homogenization of milk on particle size, water retention and texture of non-fat and low-fat yoghurts.Inter Dairy J. 2010;20:314–20. [DOI]
Mao L, Yang J, Xu D, Yuan F, Gao Y. Effects of homogenization models and emulsifiers on the physicochemical properties of β-carotene nanoemulsions.J Dispers Sci. 2010;31:986–93. [DOI]
Wang T, Wang S, Zhang L, Sun J, Guo T, Yu G, et al. Fabrication of bilayer emulsion by ultrasonic emulsification: effects of chitosan on the interfacial stability of emulsion.Ultrason Sonochem. 2023;93:10629. [DOI] [PubMed] [PMC]
Cabezas DM, Madoery R, Diehl BWK, Tomás MC. Emulsifying properties of different modified sunflower lecithins.J Am Oil Chem Soc. 2012;89:355–61. [DOI]
Liang L, Chen F, Wang X, Jin Q, Decker EA, McClements DJ. Physical and oxidative stability of flaxseed oil-in-water emulsions fabricated from sunflower lecithins: impact of blending lecithins with different phospholipid profiles.J Agric Food Chem. 2017;65:4755–65. [DOI] [PubMed]
Michalik R, Wandzik I. A mini-review on chitosan-based hydrogels with potential for sustainable agricultural applications.Polymers. 2020;12:2425. [DOI] [PubMed] [PMC]
Mengual O, Meunier G, Cayré I, Puech K, Snabre P. Turbiscan MA 2000: multiple light scattering measurement for concentrated emulsion and suspension instability analysis.Talanta. 1999;50:445–56. [DOI] [PubMed]
Xu D, Aihemaiti Z, Cao Y, Teng C, Li X. Physicochemical stability, microrheological properties and microstructure of lutein emulsions stabilized by multilayer membranes consisting of whey protein isolate, flaxseed gum and chitosan.Food Chem. 2016;202:156–64. [DOI] [PubMed]