Pretomanid

Pretomanid (1), also known as PA-824, was first identified in 1995 [13]. A key structural feature of the compound is the presence of a nitroimidazooxazine system (Figure 1). Pretomanid is an orally administrated drug that inhibits mycolic acid biosynthesis and kills actively replicating Mycobacterium tuberculosis by blocking cell wall production. It also acts as a respiratory poison and inhibits the protein synthesis of the bacteria. Pretomanid is also metabolized to form highly reactive nitrogen intermediates, including nitric oxide [14].

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Figure 1. 

Chemical structure of pretomanid and noteworthy features

Pretomanid is indicated for pulmonary XDR-TB and must be used only in combination with bedaquiline (116, see “Quinoline: bedaquiline”) and linezolid [15].

Pretomanid syntheses

Among the multitude of patents and academic articles regarding the synthesis of pretomanid [16–18], we have selected two interesting examples where we describe the divergent synthetic plans to produce the final molecule. Initial improvements for large-scale production of this compound have been proposed in the concise and convergent synthesis of PA-824 by Marsini et al. [19] in 2010.

The hallmark of their synthesis lies with the convergent synthetic design from fragments A and B, the employment of a safe imidazole derivative (8), and the use of the key chiral building block (7) derived from (R)-α-glycerol chlorohydrin (2). Briefly, the selection of a p-methoxybenzoyl group, introduced by reaction of (2) with acid chloride (3) in the first step, was key in the synthesis because of the stability and resistance to migration of this protecting group during the synthetic route. The group is removed in the final step from (9), allowing the oxazine ring construction to present in the final product by a basic treatment (Figure 2). Thus, according to synthetic Figure 2, the coupling of fragments A and B, in the form of products (7) and (8), afford the key precursor (9), which was efficiently converted into pretomanid (1) by basic treatment.

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Figure 2. 

Total synthesis of pretomanid by Marsini et al. [19]

In 2020, an efficient and practical protocol for the production of pretomanid (1) was published by Chen et al. [20], featuring the employment of cheap and readily available raw materials, mild experimental conditions, and a one-pot procedure. Key steps in this route are the selective oxirane-ring opening of (S)-epichlorohydrin (10) by the imidazole derivative (8), and the selective protection of the primary alcohol of product (12) as a silyl ether versus the contiguous secondary alcohol. The potential of the route for scale-up in an industrial capacity via this synthetic route was demonstrated by the preparation of (1) in 50 g batches on a laboratory scale (Figure 3).

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Figure 3. 

Total synthesis of pretomanid by Chen et al. [20]

Lefamulin

Lefamulin (15) is a novel semisynthetic pleuromutilin antibacterial agent and is the first antibiotic with a novel mechanism of action to obtain FDA approval for the treatment of pneumonia in nearly two decades [21]. The pleuromutilin class of antimicrobials was first discovered in the 1950s, but the first pleuromutilin was not brought to market until 1979 (tiamulin) for exclusive veterinary use. In 2006, lefamulin was synthesized and began undergoing testing for systemic human use (Figure 4) [22].

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Figure 4. 

Chemical structure of lefamulin and noteworthy chemical features

The mechanism of action of lefamulin is related to its interaction with the central part of domain V at the 23S ribosomal RNA (rRNA) through hydrophobic interactions, Van der Waal forces, and hydrogen bonds, with the side chain being the main driver for its biological activity, leading to inhibition of the biosynthesis of key bacterial proteins [23, 24].

Lefamulin syntheses

In the literature, only seven patents have been published regarding the preparation of lefamulin [25, 26]. For this review, we have decided to include here one significative route developed by Riedl and co-workers [27].

The full process is based on a semisynthetic route where the side chain is attached to pleuromutilin (16), which is a naturally ocurring antibiotic produced by basidiomycetes Pleorotus mutilus and Pleorotus passeckerianus. After the preparation of pleuromutilin (16) for the introduction of the side chain, via activation of the hydroxyl group of the 2-hydroxy acetyl unit as tosylate, mesylate group, or even as a chloride, the coupling with the enantiomerically pure thiol (23) under basic conditions in methyl-tert-butyl ether, affords the protected lefamulin (24), which is finally transformed into lefamulin in a final deprotection step.

For the synthesis of stereoisomerically pure (23), Riedl’s group [27] starts from a racemic mixture of 3-hexenoic acid (18), which is transformed into isocyanate (19) by the employment of diphenylphosphorazide. Isocyanate (19) is then treated with Cooper(I) chloride in the presence of tert-butanol to obtain the protected amine (20). Epoxidation of compound (20), using m-chloroperbenzoic acid, furnished the epoxide (21) as a mixture of enantiomers, which, after a regioselective oxirane-ring opening reaction with thiobenzoic acid, followed by a selective precipation process, provided enantiomerically pure (22), which was finally transformed into the thiol (23) in good overall yield (Figure 5).

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Figure 5. 

Semisynthetic route to achieve lefamulin by Riedl and co-workers’ patent [27]

Cephalosporin class: cefiderocol, ceftazidime, and ceftalozane

Cephalosporins, also known as carbapenems, are very well-known bactericidal β-lactam antibiotics [28]. They inhibit key bacterial enzymes (penicillin-binding proteins), including the transpeptidase enzyme, which is involved in the construction of the bacterial cell wall, and crucial for the structural strength and shape of the bacteria. Currently, there are five generations of cephalosporins.

Cefiderocol

Cefiderocol (25) is an injectable siderophore cephalosporin discovered and developed by Shionogi & Co., Ltd., Japan. As with other β-lactam antibiotics, the main antibacterial-bactericidal activity of cefiderocol is due to its inhibition of the cell wall synthesis of Gram-negative bacteria by binding to penicillin-binding proteins [29]. In 2019, cefiderocol was approved by the FDA for the treatment of cUTI, including pyelonephritis, for HABP, and for VABP. Chemically, it is related to ceftazidime (41; see “Ceftazidime”) and cefepime. The main difference structurally for cefiderocol compared to ceftazidime and cefepime is that in the 3-position of the carbapenem core, a catechol framework is present in the side chain (2-chloro-3,4-dihydroxybenzoic acid moiety) and a covalently bound pyrrolidine ring, which forms a quaternary ammonium salt (Figure 6). According to recent investigations, this side chain confers increased periplasmic concentrations of the drug due to a chelation effect between the catechol moiety with ferric iron present in the outer regions of Gram-negative bacilli, commonly known as the siderophore effect [30].

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Figure 6. 

Chemical structure of cefiderocol, a 5th-generation cephalosporin antibiotic

Cefiderocol synthesis

Among the seven patents found for the preparation of cefiderocol [31, 32], we include herein the specific synthetic route that includes the catechol framework in the cephalosporin antibacterials, registered by Shionogi & Co., Ltd. and led by Nishitani and co-workers [33]. The successful total synthesis of cefiderocol was based on the syntheses of the key moieties, fragments A, B, and C, followed by subsequent couplings to join them. As depicted in Figure 7, several steps were needed to build each scaffold independently. Thus, the acid (31) was obtained in a 49% overall yield after treatment of bromoester (26) with hydroxyphtalimide (27), which provided the nitrogen atom necessary for the subsequent imine formation. Here we point out the importance to obtain the imine compound (31) through coupling between compound (29) and ketoacid (30), which contains a free acid. On the other hand, the protected carbapenem (32), which can be readily purchased from commerical vendors was coupled with acid (31), via activation of the acid group with Mesyl (Ms) chloride in the presence of triethylamine, to furnish the amide (33) in excellent yield. Oxidation of the sulfur atom to the sulfoxide was then required to protect the sulfide group during the final coupling of compounds (35) and (40), as described later [34].

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Figure 7. 

Total synthesis of cefiderocol by Nishitani and co-workers [33]

In parallel, the preparation of catechol compound (40) required a conventional strategy of protection-deprotection reactions starting from o-chloro-acid compound (36). Having accomplished the synthesis of both coupling partners, the final key step was the alkylation of tertiary amine (40) through a bimolecular substitution reaction of iodo-compound (35). The mixture of both partners in dimethylformamide and subsequent treatment of the resulting ammonium salt with acetyl chloride and potassium iodide afforded cefideracol (25) as a white powder in a 30% yield, over two final steps (Figure 7).

Ceftazidime

Ceftazidime (41) is a third-generation injectable cephalosporin antibiotic related to the previously described cefideracol (25). Since the only difference between the compounds is the pendant side chain at the 3-carbon, we will only highlight the clinical uses and the early solution provided by Wirth [35], working at Eli Lilly and Co. to prepare the amide function using carboxylic sulfonic mixed anhydrides, a reaction that, as we have already mentioned, was later employed in the syntheses of 4th- and 5th-generation cephalosporins.

Ceftazidime was approved by the FDA in 2015 for the treatment of cUTI, for HABP, and for cIAI, where it must be used in combination with avibactam (53, see “BLI class: tazobactam, avibactam, vaborbactam, and relebactam” section), which is a BLI (Figure 8).

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Figure 8. 

Ceftazidime structure and key reaction for amide formation [35]

Ceftazidime synthesis

As we have discussed, the key reaction in the ceftazidime synthesis was the improvement introduced by Wirth [35] in the amide formation, consisting of the activation of the acid group as a mixed anhydride. Thus, compound (43) was easily prepared by treatment of acid (42) with methanesulfonyl chloride in the presence of triethylamine. Compound (43) showed no degradation over the course of several h at 0℃ and could be used directly as a solution, without the removal of by-products, which was important from the scale-up point of view. Hence, Wirth’s conditions and later modifications have been applied to the syntheses of subsequent generations of cephalosporins (Figure 8) [35].

Ceftolozane

Ceftolozane (45) is a fifth-generation cephalosporin discovered by Astellas Pharma and Wakunaga Pharmaceuticals, which has improved efficacy against resistant microorganisms [36]. Under the commercial name Zerbaxa^®, we find a combination of ceftolozane (45) and Tazobactam that is indicated to treat cIAI, cUTI, and HABP, which was approved in 2014 and 2015 by the FDA and EMA, respectively.

Ceftolozane synthesis

Since the key structural difference between ceftolozane (45) and the previously studied cephalosporins is the identity of the side chain in the 3-position of carbapenem ring, we will focus on the challenge to synthesize the pyrazole moiety.

For a full description of all published routes to obtain ceftolozane (45), we recommend the review written by Hughes [37] in 2017. We describe here the synthetic route toward the pyrazole scaffold (46). So, starting from 5-amino-1-methylpyrazole (47), the introduction of the amine group in the 4-position was achieved via a classical nitrosation reaction, followed by reduction of the nitrosocompound (48) to the bis amino compound (49). Subsequently, selective protection of the 4-amino versus the 5-amino group was accomplished by reaction with phenylchloroformate. Next protection of the 5-amino group as the trityl derivative (51) and followed by urea formation using tert-butyloxycarbonyl (Boc)-protected ethylenediamine furnished the desired pyrazole-urea (46) in a 47% overall yield (over 5 steps).

Interestingly, the final coupling of the fully functionalized carbapenem (52) and the pyrazole-urea (46) was achieved using 1,3-bis(trimethylsilyl)urea (BSU) and potassium iodide, providing the protected ceftalozane in 78% yield, in contrast to the previously discussed alternative used by Nishitani et al. [33] in the total synthesis of cefiderocol [through compounds (35) and (40), see Figure 7]; (Figure 9).

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Figure 9. 

Ceftolozane, synthesis of side chain and key reaction for amide formation

Avibactam

Avibactam (53) is a BLI, which in combination with ceftazidime (41, Zavicefta^®) has been approved by the EMA in 2015 for the treatment of cIAI, cUTI, and HABP [40].

From the structural point of view, avibactam (53) is comprised of a bicyclic urea core of 5- and 6-membered fused rings that contain an amide functional group and a sodium sulfate as pendants groups at the C-2 and N-6 positions, respectively (Figure 10).

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Figure 10. 

Chemical structure of avibactam and noteworthy chemical features

Avibactam synthesis

Syntheses of avibactam (53) have remained unchanged throughout its development history. We will discuss here the optimized route currently in practice (Figure 11) [40].

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Figure 11. 

Performed avibactam industrial synthesis

Starting from the glutamic acid derivative (54), the key intermediate (58) was obtained in good overall yield according to the synthetic sequence in which the resulting products (55–57) were not isolated, which is important from the industrial point of view, as it avoids the isolation of reactive intermediates. Opening of the pyrrolidine ring using trimethylsulfoxonium iodide in basic media was followed by oxime formation and displacement of the chlorine atom to yield intermediate (57), which was stereoselectively reduced by treatment with sodium triacetoxyborohydride. The product was then cleanly crystallized as an oxalate salt by using a ternary solvent mixture consisting of methanol isopropyl alcohol (IPA)-ethyl acetate to obtain the salt (58) [41].

Then, after a series of selective protection-deprotection reactions of (58) and the coupling of the resulting product (59) with the imidazole acid chloride (60), the introduction of the sulfate group in compound (62) was successfully achieved by the employment of a sulfur trioxide-trimethylamine complex. Finally, the cation exchange of the tetrabutylammonium counterion with sodium was achieved, employing sodium 2-ethylhexanoate (Figure 11).

Relebactam

Relebactam (64) is a structural analogue of avibactam (53) approved in 2019 and used in combination with imipenem and cilastatin for the treatment of cUTI, cIAI, and HABP/VABP. The only difference between the antibiotics is that relebactam contains a 4-amino piperidine in the pendant amide group (Figure 12) [41].

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Figure 12. 

Chemical structure of relebactam, an analogue of avibactam

Relebactam synthesis

Despite the structural core of relebactam being identical to avibactam (53), we have selected here an early alternative route to obtain relebactam (64) that highlights the versatility of organic synthesis to achieve quite similar objectives (Figure 13) [42].

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Figure 13. 

Early total synthesis of relebactam by Mangion’s group [42]

Thus, Mangion’s group [42] published in 2011 a concise and multi-kilogram scalable synthesis of relebactam to supply clinical trials. The synthesis utilizes the inexpensive and readily available starting material, L-pyroglutamic acid (65). The first steps are quite similar to those used in the avibactam synthesis, most notably the ring expansion via sulfoxonium ylide formation [43]. We highlight here, a novel iridium-catalyzed N-H insertion reaction to afford the chiral core 3-piperidinone moiety (67). Then, a diastereoselective ketone reduction followed by alcohol activation and displacement with a hydroxyl amine derivative furnished the compound (70) in good overall yield. Triphosgene-mediated urea formation provided the bicyclo[3.2.1]urea (71), which through functional group interconversion, yielded relebactam (64, Figure 13).

Vaborbactam

Vaborbactam (73) is a new BLI featuring acyclic boronic acid pharmacophore approved in 2017 to be used in combination with meropenem (a penicillin compound derivative), indicated for the treatment of cUTI and pyelonephritis [44].

Chemically, vaborbactam (73) is unique because it is the only antibiotic approved thus far in the last decade that contains a boronic acid as a pharmacophore. Furthermore, it contains an amide and a thiophene group (Figure 14).

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Figure 14. 

Vaborbactam contains a boronic acid as pharmacophore

The initial reports of aryl boronic acids as BLIs were in 1978 by Kiener and Waley [45]. Since then, only a few examples in the literature have been reported, with vaborbactam being of the biggest significance [46, 47].

Vaborbactam synthesis

Among the syntheses reported of vaborbactam (73) [48, 49], we shall discuss here a recent development that highlights the importance of continuous flow chemistry for the production of pharmaceutical compounds (Figure 15) [50]. Specifically, the development of the Matteson reaction for full-scale production of the important intermediate (77) reported by Stueckler et al. [51] and Hughes [52] represents a key breakthrough in the field of flow chemistry. Therefore, starting from the racemic pentanoate (74), and after classical multi-step processes, boronic compound (75) was obtained as an enantiomerically pure compound. Transesterification using (+)-pinanediol afforded the precursor for the Matteson reaction [53], compound (76). The flow chemistry design facilitated the homologation reaction to provide the chloro compound (77), which can participate in a bimolecular substitution reaction with the corresponding amide in basic media to form the compound (78), which is finally converted to the final product (73) by acidic media and heating.

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Figure 15. 

Flow chemistry used in the total synthesis of vaborbactam (73)

Eravacycline

Eravacycline (79) is the first fully synthetic fluorocycline that has been approved by the FDA in 2018 for the treatment of cIAIs. Chemically, it is similar to the newly developed semisynthetic tetracycline, tigecycline (80) [55].

Structure-activity relationship (SAR) studies of the fluorocyclines have shown that more polar or basic substituents attached to the C-9 position, such as the pyrrolidine analogue in the case of eravacycline (79), result in improved antibacterial activity. Specifically, in case of eravacycline, which possesses a pyrrolidine moiety, exhibited 4–64-fold more potency versus other derivatives with substituents such as azetidine or piperidine (Figure 16) [55].

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Figure 16. 

Eravacycline is the first fully synthetic fluorocycline on the market

Eravacycline synthesis

While almost all these complex antibiotics are prepared by semisynthesis, Charest et al. [56] published in 2005 a general, enantioselective, and convergent route to access a group of tetracyclines with the D-ring as a site of structural diversity. Taking advantage of the platform designed by Ronn and co-workers [55], working at Tetraphase Pharmaceuticals, achieved in 2013 the synthesis of a series of 7-fluoro-9-substituted tetracycline analogues, including the total synthesis of eravacycline (79).

Taking advantage of the oxidative enzymes in the organism Alcaligenes eutrophus B9, the benzoic acid (81) was converted to the enantioenriched dihydroxy acid (82), which was regioselectively epoxidized using meta-chloroperbenzoic acid to provide compound (83). After esterification of (82), the resulting ester was treated with TBSOTf resulting in the isomerization of the vinylogous epoxy alcohol and silyl protection of the resulting diol to afford compound (84). In order to construct the A-ring, compound (84) was treated with the lithium derivative (85), and the resulting ketone (86) subjected to an intramolecular oxirane-ring opening by treatment with lithium triflate at 60℃ to obtain (87). The last steps were addressed to stabilize the α,β-unsaturated ketone in the B-ring and entailed isomerization of a double bond and further oxidation of corresponding alcohol to obtain the precursor of the Michael-Dieckmann reaction, compound (89).

Thus, a Michael-Dieckmann reaction [57] between the tricyclic compound (89) and fluoroderivative (90) was employed to form the C-ring of the tetracycline core. A key feature of this chemical transformation is the high degree of stereoselectivity exhibited, yielding the scaffold (91) in 94% of yield. Two additional steps of protecting group removal provided the fully deprotected compound (92), which was reacted with acid chloride (93) to form the amide and form eravacyclice (79). Finally, the free base was converted to its hydrochloride salt (Figure 17).

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Figure 17. 

Total synthesis of eravacycline (79), the first fully synthetic fluorocycline

Sarecycline

Sarecycline (94) is a new oral tetracycline-class antibiotic developed by Paratek and Allergan [58]. Structurally, sarecycline is a 7-substituted [[methoxy(methyl)amino]-methyl] tetracycline (7-substituted sancycline, Figure 18). It was approved by the FDA in 2018 for inflammatory lesions of non-nodular moderate to severe acne vulgaris. This improved activity may be due to higher lipophilicity at physiologically relevant pH, allowing for better penetration of the compound into the lipid-rich sebaceous follicular tissue [59].

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Figure 18. 

Sarecycline is a 7-substituted sancycline

Sarecycline synthesis

All reported methods to synthesize sarecycline (94) are intellectually protected by patents [60]. Among them, the most interesting method corresponds to the preparation of sarecycline via semisynthesis starting from sancycline (95) [59], which can be prepared by various routes, such as those utilizing 6-demethyltetracyclines by esterification with formic acid, followed by hydrogenolysis [60]. Thus, iodination of sancycline introduces an iodine atom in the C-7 position of the D-ring, which is accomplished by reaction with N-iodosuccinimide (NIS) in the presence of trifluoroacetic acid to obtain (96). Then, a palladium catalyzed formylation introduces the formyl group (compound 97) using bis-triphenylphosphinepalladium dichloride as the catalyst. Compound (97) is then subjected to a reductive amination employing dimethylhydroxylamine (DMHA) and dimethylaminoborane in the presence of oxalic acid to form the oxalate salt (98). The final steps are neutralization and salt formation using hydrochloride acid (Figure 19).

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Figure 19. 

Semisynthesis of sarecycline (94) patented by Giovanni et al. [59]

Omadacycline

Omadacycline (100) was discovered in 2015 in a full study of the SARs of aminomethylcycline (AMC) by scientists working at Paratek Pharmaceuticals (Boston, USA) [61]. The compound was approved by the FDA in 2018 to treat CABP and ABSSSI (Figure 20).

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Figure 20. 

Omadacycline is an AMC discovered in an SAR-study

Omadacycline synthesis

For the synthesis of omadacycline (100), the route for its preparation uses a semisynthesis, starting from minocycline (101), as it possesses the key structural framework required for activity. So, from (101), iodination in the of silver sulfate in acidic media [62] introduces an iodine atom in the D-ring, which is then converted to a formyl group utilizing carbon monoxide and palladium(0) catalysis [63] to afford (102), which participates in a conventional reductive amination reaction with isobutylamine to provide omadacycline (100, Figure 21).

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Figure 21. 

Semisynthesis of omadacycline from minocycline

Delafloxacin

Delafloxacin (103) is a fluoroquinolone approved by the FDA in 2017 to treat ABSSSI and CABP. Delafloxacin has shown greater antibacterial efficicacy than other fluoroquinolones, but maintains the same inhibitory activity against bacterial DNA topoisomerase. The improved biological action is due to the appended groups introduced at the N-1, C-7, and C-8 positions. The azetidine substitution at C-7 results in a weak acid, resulting in an increase in its activity in an acidic medium. The chlorine in C-8 is an electron-withdrawing group, reducing the reactivity of the heterocycle and stabilizing the molecule, and finally, the difluoropyridine substitution at N-1 increases the molecular surface versus other quinolones (Figure 23) [65].

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Figure 23. 

Chemical features of new fluoroquinolone delafloxacin

Delafloxacin synthesis

Delafloxacin, also known as ABT-492, was licensed by Abbott Labs. Scientists at Abbott have described two related ways to synthesize delafloxacin [66]. Herein we report the most interesting pathway, which starts with a reaction between tetrafluoropyridine (104) with benzylamine (105) at reflux (ortho-amination reaction is a key step). Then, compound (106) is debenzylated with hydrogen over Pd/C in methanol to yield the amine (107). Reaction of amine (107) with 4-methoxybenzylamine (108) in N-methylpyrrolidone at 140°C affords compound (109, another ortho-amination reaction is key for the introduction of nitrogen atoms in suitable positions), which is then coupled with 2-(3-chloro-2,4,5-trifluorobenzoyl)-3-ethoxyacrylic acid ethyl ester (111) to yield (112). Ester (111) is obtained by condensation of 2-(3-chloro-2,4,5-trifluorobenzoyl)acetic acid ethyl ester (110) with triethyl orthoformate and acetic anhydride. Final deprotection and attachment of the hydroxy azetidine sidechain provide delafloxacin (103, Figure 24).

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Figure 24. 

Synthesis of delafloxacin by Abbot Labs [66]

Ozenoxacin

Ozenoxacin (113) is a quinolone approved by the FDA in 2017 for topical treatment of impetigo due to Staphylococcus aureus or Streptococcus pyogenes. Impetigo is one of the most common bacterial skin infections in children, particularly between the ages of 2–5 and can be classified as an ABSSSI primarily affecting the uppermost layers of the epidermis [67].

Ozenoxacin synthesis

To date, there are not publications from academic labs regarding the synthesis of ozenoxacin, however, several patents covering its preparation are published [68]. One of them employs the Suzuki reaction [69] as the key step transformation in the cross-coupling of two fragments as depicted in Figure 25, the boronic acid (114) and advanced intermediate (115) [70].

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Figure 25. 

The chemical features and key step of ozenoxacin synthesis

Bedaquiline syntheses

For a comprehensive review covering all reported syntheses of bedaquiline, we strongly recommend the excellent article written by Calvert and co-workers [71] in 2020, where bedaquiline and several derivatives are discussed. Nevertheless, we highlight the industrial synthesis of bedaquiline because of its low cost of preparation, and this is despite the fact that the route affords large amounts of undesired bedaquiline isomers. The synthesis begins with bromoquinoline (117), which is treated with lithium diisopropylamide to generate the corresponding carbanion, which then attacks the electrophilic ketone (118), providing quantitatively compound (119), but without any stereoselectivity. Further resolution of ammonium salt (122), obtained after treatment of (120) with phosphoric acid (121), allows the isolation of the isomer with the correct stereochemistry. Finally, basification employing potassium carbonate affords bedaquiline (116) a modest 39% yield (Figure 27).

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Figure 27. 

Industrial synthesis of bedaquiline

Plazomicin synthesis

Plazomicin (124) has been prepared by semisynthesis. Sisomicin sulfate (126) was rendered basic by treatment with an ion exchange resin, followed by a reaction with ethyl trifluorothioacetate to selectively form the trifluoroacetamide (127). Treatment of compound (127) with Zn(II) acetate and benzyloxycarbonyl (Z) succinimide results in selective protection of both the 2’ and 3’ positions to form compound (128). N-1 acylation was achieved by reaction with the active ester (129) to yield the corresponding amide, which was deprotected using concentrated ammonia to afford compound (130). Reductive amination of (130), affords compound (131), and final protecting group removals provide plazomicin (124) in poor yield, taking into consideration that several steps required high performance liquid chromatography (HPLC) purification of intermediates (Figure 29) [75].

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Figure 29. 

Semisynthesis of plazomicin by Aggen’s group [75]

Tedizolid syntheses

The main route for the preparation of tedizolid was patented by Trius Therapeutics and is depicted in Figure 33 [80]. This synthesis starts from bromocianopyridine (137), which after reaction with sodium azide in the presence of ammonium chloride at 90℃ yields the tetrazole derivative (138) in the form of the ammonium salt. This compound is then methylated using methyl iodide. Sequential treatment with 6 mol/L hydrochloric acid and sodium hydroxide to basic pH and final recrystallization in isopropyl acetate affords the compound (139). Then, again a Suzuki coupling reaction is required as a key step to connect the two advanced precursors, compound (139) and boronic acid (142). Deprotonation of the carbamate within (143) using the strong base lithium bis(trimethylsilyl)amide, followed by reaction with R-(–)-glycidyl butyrate (144) in the presence of DMPU, generates tedizolid (136) in 85% yield through an opening of the oxirane ring of (144) and subsequent cyclization of the ring-opened intermediate. Finally, a phosphate formation reaction using phosphorous(V) oxychloride at 0℃ to provides tedizolide phosphate (135) in a good yield of 76% (Figure 33).

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Figure 33. 

Patented route by Trius Therapeutics to synthesize tedizolid [80]

Additionally, Im et al. [81] published in 2011 a similar synthesis of tedizolid, but using a coupling protocol with a tributyltin derivative and Pd(II) utilizing a classical Stille coupling reaction as the key step [82].